《植物生理学报》 2016, 52(3): 349-355

黄花倒水莲的组织培养和快速繁殖

杨国¹, 罗洁³, 莫亿伟¹, 陈红锋^2,*

¹绍兴文理学院生命科学院, 浙江绍兴312000; ²中国科学院华南植物园, 广州510650; ³绍兴文理学院元培学院, 浙江绍兴312000

通信作者：陈红锋；E-mail: h.f.chen@scbg.ac.cn

摘　要：

以黄花倒水莲无菌实生苗的胚轴、茎段和叶片为外植体, 研究了不同植物生长调节剂对不同外植体的不定芽诱导的影响, 以及光照、有机添加物对不定芽增殖和生长的影响。结果表明: 培养基MS+BAP 2.0 μmol•L^-1+NAA 0.2 μmol•L^-1最利于茎段和胚轴诱导不定芽发生; 叶片在MS+2,4-D 4.0 μmol•L^-1培养基上培养30 d可诱导出愈伤组织, 转入MS+BAP 2.0 μmol•L^-1+NAA 0.2 μmol•L^-1培养基中培养20 d能分化成不定芽。当培养光照达到100 μmol•m^-2•s^-1时, 最利于黄花倒水莲芽苗生长, 培养基中分别添加1 g•L^-1蛋白胨、50 g•L^-1椰汁和50 g•L^-1香蕉, 均有壮苗的效果; 最佳的生根培养基是MS+2,4-D 0.5 μmol•L^-1, 生根率达95.3%, 且根系生长良好; 移栽到混合基质(蚯蚓粪:黄泥土=2:1)中, 生长良好, 成活率达95%。

关键词：黄花倒水莲; 药用植物; 离体快繁; 组织培养

收稿：2015-12-25   修定：2016-01-31

资助：广东省林业科技创新项目(2015KJCX037)和绍兴文理学院校级项目。

Tissue culture and rapid propagation in vitro of Polygala fallax

YANG Guo¹, LUO Jie³, MO Yi-Wei¹, CHEN Hong-Feng^2,*

¹Shaoxing University, Academy of Life Science, Shaoxing, Zhejiang 312000, China; ²South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; ³Shaoxing University, Academy of Yuanpei, Shaoxing, Zhejiang 312000, China

Corresponding author: CHEN Hong-Feng; E-mail: h.f.chen@scbg.ac.cn

Abstract:

The regeneration system of tissue culture of Polygala fallax was established by utilizing its in vitro-derived hypocotyl, stem and leaf as explants. We analyzed the differentiations characteristics of different explants, and discussed affecting factors of tissue culture of hormone, light and additional nutrient. The results showed that MS+BAP 2.0 μmol•L^-1+NAA 0.2 μmol•L^-1 was most suitable for adventitious buds induction from stem and hypocotyl explants, the callus were induced on MS+2,4-D 4.0 μmol•L^-1 from the leaf explants, and achieved 54.4% induction rate after 30 days, adventitious buds appeared from the surface of callus induced on MS+BAP 2.0 μmol•L^-1+NAA 0.2 μmol•L^-1 after 20 days. The plantlets grew well when the light was 100 μmol•m^-2•s^-1, 1 g•L^-1 peptone, 50 g•L^-1 coconut juice and 50 g•L^-1 banana were good for cultivating strong seedling. The best rooting medium was MS+2,4-D 0.5 μmol•L^-1, the rooting rate has reached 95.3%, Plantlets were transplanted to a potting mixture (2:1, wormcast: yellow mud) in trays with about 95% survival percentage.

Key words: Polygala fallax; medicinal plant; tissue culture; rapid propagation in vitro